卵泡发育相关基因在牛优势和从属卵泡颗粒细胞中表达的研究

doi:10.11843/j.issn.0366-6964.2014.12.007

Abstract

Abstract:

To study the regulatory mechanism of the relevant genes associated with follicular development，we test the genes expression difference between the dominant follicles (DF) and subordinate follicles (SF) granulose cells of the first follicular wave during estrous cycle in bovine.Bioinformatics methods were used to screen the candidate genes associated with follicular development，and real-time fluorescent quantitative PCR was performed to detect the gene expression pattern between DF and SF granulose cells of the first follicular wave during estrous cycle in bovine.The results showed that 6 candidate genes (SFRP2，CPEB1，PRKAG2，MAPK8,PPP2R2A and PRSS23) related to follicular development were screened.CPEB1 expressed in DF was extremely significantly higher than that in SF (P<0.01)，and PRKAG2 mRNA amounts were significantly greater in DF compared with SF (P<0.05).But the expression levels of SFRP2 and MAPK8 were extremely significantly higher in SF than those in DF (P<0.01).CPEB1 and PRKAG2 may play a crucial role to promote the follicular development，however，SFRP2 and MAPK8 may inhibit the follicular development in bovine.

CLC Number:

S823.2

YAO Xiao-lei,LI Peng-fei，JIANG Xiao-long，MENG Jin-zhu,HUANG Yang，Lü Yan，CHEN Jian-wei,ZHAO Miao-miao,LYU Li-hua. Research of the Genes Expression Associated with Follicular Development in Bovine Dominant and Subordinate Follicle Granulose Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1957-1963.

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Research of the Genes Expression Associated with Follicular Development in Bovine Dominant and Subordinate Follicle Granulose Cells

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